منابع مشابه
An improved and simplified radioisotopic assay for the determination of free and esterified carnitine.
The radioisotopic assay for carnitine first described by Cederblad and Lindstedt (Clin. Chim. Acta. 37:235-543, 1972) and modified by Bohmer et al (Clin. Chim. Acta. 57:55-61, 1974) has been improved and simplified. As a result, the assay yields a linear response over a wide range of carnitine concentrations without the need for excessive amounts of labeled acetyl-CoA. In addition, it will meas...
متن کاملCarnitine: determination of total carnitine using a radioenzymatic assay.
Acetylcarnitine and acylcarnitine are hydrolyzed by alkaline hydrolysis. The total carnitine is measured by incubation of the sample with 14CacetylCoA and carnitine acetyltransferase. The radioactive acetylcarnitine is separated from the unreacted radioactive acetylCoA using a Dowex 2 x 8 anion exchange column. The radioactive acetylcarnitine fraction is quantitated by counting in a liquid scin...
متن کاملRadioisotopic assay for measurement of serum folate levels.
A radioisotopic assay is reported that 4 hr and can be run in unlimited quanaccurately measures an almost identical tity. This raclioassay should simplify diserum folate to that measured by Lactoagnosis of folate deficiency and be parbacillus casei and can separate low, ticularly useful in patients receiving borderline, and normal folate levels. This antibiotics and folate antagonists, where as...
متن کاملComparison of flow cytometry and immunohistochemistry in non-radioisotopic murine lymph node assay using bromodeoxyuridine.
Non-radioisotopic local lymph node assay (LLNA) employing 5-bromo-2'-deoxyuridine (BrdU) with flow cytometry (FACS) or immunohistochemistry (IHC) is gaining attention due to a regulatory issue of using radioisotope, (3)H-thymidine, in vivo in traditional LLNA. In this study, to compare the performance of these non-radioisotopic endpoints, 7 chemicals with known sensitizing potencies were examin...
متن کاملNoninvasive Radioisotopic Technique
At 8 and 32 hours after saphenous vein bypass graft surgery in six dogs, in vivo images of the graft were obtained with a gamma camera after intravenous injection, 2 hours postoperatively, of autologous platelets labeled with indium-111. Platelet deposition in the grafts could be imaged in vivo from the scintiphotos. In vitro images of the excised heart showed saphenous vein graft uptake confir...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Protocol Exchange
سال: 2011
ISSN: 2043-0116
DOI: 10.1038/protex.2011.240